Methods and Materials
In this section you describe how you performed your study. You need to provide enough information here for the reader to duplicate your experiment. However, be reasonable about who the reader is. Assume that he or she is someone familiar with the basic practices of your field.
It's helpful to both writer and reader to organize this section chronologically: that is, describe each procedure in the order it was performed. For example, DNA-extraction, purification, amplification, assay, detection. Or, study area, study population, sampling technique, variables studied, analysis method.
Include in this section:- study design: procedures should be listed and described, or the reader should be referred to papers that have already described the used procedure
- particular techniques used and why, if relevant
- modifications of any techniques; be sure to describe the modification
- specialized equipment, including brand-names
- temporal, spatial, and historical description of study area and studied population
- assumptions underlying the study
- statistical methods, including software programs